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b4galt1 grx1 rogfp1 ie gene sequence  (Addgene inc)


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    Addgene inc b4galt1 grx1 rogfp1 ie gene sequence
    B4galt1 Grx1 Rogfp1 Ie Gene Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b4galt1+genes/B4GALT1-Dox+(Plasmid+%23124633)/pmc11904595__mmc1-10-20-26
    Average 93 stars, based on 2 article reviews
    b4galt1 grx1 rogfp1 ie gene sequence - by Bioz Stars, 2026-10
    93/100 stars

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    Sequencing:

    Article Title: Contemporary human H3N2 influenza A viruses require a low threshold of suitable glycan receptors for efficient infection
    Article Snippet: .. The B3GNT2 and B4GALT1 genes were always proceeded by the signal sequence of the human GalT, which we copied from the EGFP-GalT plasmid (gift from Jennifer Lippincott-Schwartz, Addgene plasmid # 11929; ). .. The T2A self-cleaving peptide was amplified from plasmid tetO.Sox9.Puro , which was a gift from Henrik Ahlenius (Addgene plasmid # 117269).

    Article Title: Contemporary human H3N2 influenza A viruses require a low threshold of suitable glycan receptors for efficient infection
    Article Snippet: .. The B3GNT2 and B4GALT1 genes were always proceeded by the signal sequence of the human GalT, which we copied from the EGFP-GalT plasmid (gift from Jennifer Lippincott-Schwartz, Addgene plasmid # 11929) [ ]. .. The T2A self-cleaving peptide was amplified from plasmid tetO.Sox9.Puro [ ], which was a gift from Henrik Ahlenius (Addgene plasmid # 117269).

    Article Title: Contemporary human H3N2 influenza A viruses require a low threshold of suitable glycan receptors for efficient infection.
    Article Snippet: .. The B3GNT2 and B4GALT1 genes were always proceeded by the signal sequence of the human GalT, which we copied from the EGFP-GalT plasmid (gift from Jennifer LippincottSchwartz, Addgene plasmid # 11929; Cole et al. 1996). .. The T2A self-cleaving peptide was amplified from plasmid tetO.Sox9.Puro (Canals et al. 2018), which was a gift from Henrik Ahlenius (Addgene plasmid # 117269).

    Plasmid Preparation:

    Article Title: Contemporary human H3N2 influenza A viruses require a low threshold of suitable glycan receptors for efficient infection
    Article Snippet: .. The B3GNT2 and B4GALT1 genes were always proceeded by the signal sequence of the human GalT, which we copied from the EGFP-GalT plasmid (gift from Jennifer Lippincott-Schwartz, Addgene plasmid # 11929; ). .. The T2A self-cleaving peptide was amplified from plasmid tetO.Sox9.Puro , which was a gift from Henrik Ahlenius (Addgene plasmid # 117269).

    Article Title: Contemporary human H3N2 influenza A viruses require a low threshold of suitable glycan receptors for efficient infection
    Article Snippet: .. The B3GNT2 and B4GALT1 genes were always proceeded by the signal sequence of the human GalT, which we copied from the EGFP-GalT plasmid (gift from Jennifer Lippincott-Schwartz, Addgene plasmid # 11929) [ ]. .. The T2A self-cleaving peptide was amplified from plasmid tetO.Sox9.Puro [ ], which was a gift from Henrik Ahlenius (Addgene plasmid # 117269).

    Article Title: Contemporary human H3N2 influenza A viruses require a low threshold of suitable glycan receptors for efficient infection.
    Article Snippet: .. The B3GNT2 and B4GALT1 genes were always proceeded by the signal sequence of the human GalT, which we copied from the EGFP-GalT plasmid (gift from Jennifer LippincottSchwartz, Addgene plasmid # 11929; Cole et al. 1996). .. The T2A self-cleaving peptide was amplified from plasmid tetO.Sox9.Puro (Canals et al. 2018), which was a gift from Henrik Ahlenius (Addgene plasmid # 117269).

    other:

    Article Title: Chimeric antigen receptor T cells targeting the GM3(Neu5Gc) ganglioside.
    Article Snippet: T cells were activated by a 48-hour incubation in anti-CD3/ anti-CD28 mAb-coated cell culture plates and then transduced by a 30-minute centrifugation with retrovirus-containing supernatant in 24-well plates pre-coated with RetroNectin (Clontech Laboratories, CA, USA).



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    Enzyme assays of KS biosynthetic enzymes toward linear and branched O -Man glycans . A , enzymes involved in the KS biosynthesis. B , qPCR analysis of <t>B4galt1</t> , B4galt4 , B3gnt7 , Chst1 , Chst2 and Chst5 mRNAs in GnT-IX-KO mouse brains (P0) was performed, and their relative levels normalized by that of Gapdh are shown as a graph (n = 3, mean ± SD, ∗ p < 0.05, ∗∗ p < 0.01, unpaired t test). C , soluble B4GALT1, B4GALT4, B3GNT7, CHST1, CHST2, and CHST6 were expressed in HEK293 T cells and purified from the media using a Ni2+ column. The purified enzymes were separated by SDS-PAGE and visualized by CBB staining. D – F , Purified B4GALT1, B4GALT4, B3GNT7, CHST1, CHST2, and CHST6 were incubated with GnM-S-Flu or Gn(Gn)M-S-Flu (for B4GALT1 and B4GALT4) ( D ), GalGnM-S-Flu or GalGn(GalGn)M-S-Flu (for B3GNT7) ( E ), or GnGalGnM-S-Flu or GnGalGn(GnGalGn)M-S-Flu (for CHSTs) ( F ), and the reaction mixtures were analyzed by reverse-phase HPLC. The amount of CHST1 used for GnGalGn(GnGalGn)M-S-Flu was one-20th of the amount for GnGalGnM-S-Flu, while the same amounts of the other enzymes were used for linear and branched substrates. The positions of sulfate residue were predicted by the substrate specificity of the enzymes. The specific activities were calculated from the peak areas (n = 8–17, mean ± SD, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, Holm-Sidak’s multiple comparison test for B4GALT1, B4GALT4, CHST1, CHST2, and CHST6, and unpaired t test for B3GNT7).
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    Enzyme assays of KS biosynthetic enzymes toward linear and branched O -Man glycans . A , enzymes involved in the KS biosynthesis. B , qPCR analysis of <t>B4galt1</t> , B4galt4 , B3gnt7 , Chst1 , Chst2 and Chst5 mRNAs in GnT-IX-KO mouse brains (P0) was performed, and their relative levels normalized by that of Gapdh are shown as a graph (n = 3, mean ± SD, ∗ p < 0.05, ∗∗ p < 0.01, unpaired t test). C , soluble B4GALT1, B4GALT4, B3GNT7, CHST1, CHST2, and CHST6 were expressed in HEK293 T cells and purified from the media using a Ni2+ column. The purified enzymes were separated by SDS-PAGE and visualized by CBB staining. D – F , Purified B4GALT1, B4GALT4, B3GNT7, CHST1, CHST2, and CHST6 were incubated with GnM-S-Flu or Gn(Gn)M-S-Flu (for B4GALT1 and B4GALT4) ( D ), GalGnM-S-Flu or GalGn(GalGn)M-S-Flu (for B3GNT7) ( E ), or GnGalGnM-S-Flu or GnGalGn(GnGalGn)M-S-Flu (for CHSTs) ( F ), and the reaction mixtures were analyzed by reverse-phase HPLC. The amount of CHST1 used for GnGalGn(GnGalGn)M-S-Flu was one-20th of the amount for GnGalGnM-S-Flu, while the same amounts of the other enzymes were used for linear and branched substrates. The positions of sulfate residue were predicted by the substrate specificity of the enzymes. The specific activities were calculated from the peak areas (n = 8–17, mean ± SD, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, Holm-Sidak’s multiple comparison test for B4GALT1, B4GALT4, CHST1, CHST2, and CHST6, and unpaired t test for B3GNT7).
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    Enzyme assays of KS biosynthetic enzymes toward linear and branched O -Man glycans . A , enzymes involved in the KS biosynthesis. B , qPCR analysis of <t>B4galt1</t> , B4galt4 , B3gnt7 , Chst1 , Chst2 and Chst5 mRNAs in GnT-IX-KO mouse brains (P0) was performed, and their relative levels normalized by that of Gapdh are shown as a graph (n = 3, mean ± SD, ∗ p < 0.05, ∗∗ p < 0.01, unpaired t test). C , soluble B4GALT1, B4GALT4, B3GNT7, CHST1, CHST2, and CHST6 were expressed in HEK293 T cells and purified from the media using a Ni2+ column. The purified enzymes were separated by SDS-PAGE and visualized by CBB staining. D – F , Purified B4GALT1, B4GALT4, B3GNT7, CHST1, CHST2, and CHST6 were incubated with GnM-S-Flu or Gn(Gn)M-S-Flu (for B4GALT1 and B4GALT4) ( D ), GalGnM-S-Flu or GalGn(GalGn)M-S-Flu (for B3GNT7) ( E ), or GnGalGnM-S-Flu or GnGalGn(GnGalGn)M-S-Flu (for CHSTs) ( F ), and the reaction mixtures were analyzed by reverse-phase HPLC. The amount of CHST1 used for GnGalGn(GnGalGn)M-S-Flu was one-20th of the amount for GnGalGnM-S-Flu, while the same amounts of the other enzymes were used for linear and branched substrates. The positions of sulfate residue were predicted by the substrate specificity of the enzymes. The specific activities were calculated from the peak areas (n = 8–17, mean ± SD, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, Holm-Sidak’s multiple comparison test for B4GALT1, B4GALT4, CHST1, CHST2, and CHST6, and unpaired t test for B3GNT7).
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    b4galt1 grx1 rogfp1 ie gene sequence  (Addgene inc)
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    Addgene inc b4galt1 grx1 rogfp1 ie gene sequence
    Enzyme assays of KS biosynthetic enzymes toward linear and branched O -Man glycans . A , enzymes involved in the KS biosynthesis. B , qPCR analysis of <t>B4galt1</t> , B4galt4 , B3gnt7 , Chst1 , Chst2 and Chst5 mRNAs in GnT-IX-KO mouse brains (P0) was performed, and their relative levels normalized by that of Gapdh are shown as a graph (n = 3, mean ± SD, ∗ p < 0.05, ∗∗ p < 0.01, unpaired t test). C , soluble B4GALT1, B4GALT4, B3GNT7, CHST1, CHST2, and CHST6 were expressed in HEK293 T cells and purified from the media using a Ni2+ column. The purified enzymes were separated by SDS-PAGE and visualized by CBB staining. D – F , Purified B4GALT1, B4GALT4, B3GNT7, CHST1, CHST2, and CHST6 were incubated with GnM-S-Flu or Gn(Gn)M-S-Flu (for B4GALT1 and B4GALT4) ( D ), GalGnM-S-Flu or GalGn(GalGn)M-S-Flu (for B3GNT7) ( E ), or GnGalGnM-S-Flu or GnGalGn(GnGalGn)M-S-Flu (for CHSTs) ( F ), and the reaction mixtures were analyzed by reverse-phase HPLC. The amount of CHST1 used for GnGalGn(GnGalGn)M-S-Flu was one-20th of the amount for GnGalGnM-S-Flu, while the same amounts of the other enzymes were used for linear and branched substrates. The positions of sulfate residue were predicted by the substrate specificity of the enzymes. The specific activities were calculated from the peak areas (n = 8–17, mean ± SD, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, Holm-Sidak’s multiple comparison test for B4GALT1, B4GALT4, CHST1, CHST2, and CHST6, and unpaired t test for B3GNT7).
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    b4galt1 genes  (Addgene inc)
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    FIGURE 1 GM3(Neu5Gc) synthesis and expression in murine cell lines. (A) Schematic overview of the ganglioside biosynthesis pathway. Genes names encoding the enzymes responsible for conversion steps are given in red. Created with BioRender. (B) Drawing illustrating the hydroxylation of N-acetylneuraminic acid (Neu5Ac) to N-glycolylneuraminic acid (Neu5Gc), catalyzed by cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH). (C, D) Histograms showing surface 14F7 scFv staining in wild type (WT) and Cmah -, St3gal5-, and <t>B4galt1</t> knock-out variants of the murine plasmacytoma cell line X63 and the B lymphoma cell line L1210. (E) Histograms showing 14F7 scFv staining of murine cell lines under standard cell culturing conditions. (F) Histograms showing 14F7 scFv staining of murine colon cancer CT26 and melanoma B16F10 (B16) cells ex vivo isolated from established s.c. tumors (>5mm diameter). (G) 14F7 mAb immunohistochemistry staining of established NS0 and B16 tumors (>5mm diameter). Scale bars indicate 100 µm.
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    Enzyme assays of KS biosynthetic enzymes toward linear and branched O -Man glycans . A , enzymes involved in the KS biosynthesis. B , qPCR analysis of B4galt1 , B4galt4 , B3gnt7 , Chst1 , Chst2 and Chst5 mRNAs in GnT-IX-KO mouse brains (P0) was performed, and their relative levels normalized by that of Gapdh are shown as a graph (n = 3, mean ± SD, ∗ p < 0.05, ∗∗ p < 0.01, unpaired t test). C , soluble B4GALT1, B4GALT4, B3GNT7, CHST1, CHST2, and CHST6 were expressed in HEK293 T cells and purified from the media using a Ni2+ column. The purified enzymes were separated by SDS-PAGE and visualized by CBB staining. D – F , Purified B4GALT1, B4GALT4, B3GNT7, CHST1, CHST2, and CHST6 were incubated with GnM-S-Flu or Gn(Gn)M-S-Flu (for B4GALT1 and B4GALT4) ( D ), GalGnM-S-Flu or GalGn(GalGn)M-S-Flu (for B3GNT7) ( E ), or GnGalGnM-S-Flu or GnGalGn(GnGalGn)M-S-Flu (for CHSTs) ( F ), and the reaction mixtures were analyzed by reverse-phase HPLC. The amount of CHST1 used for GnGalGn(GnGalGn)M-S-Flu was one-20th of the amount for GnGalGnM-S-Flu, while the same amounts of the other enzymes were used for linear and branched substrates. The positions of sulfate residue were predicted by the substrate specificity of the enzymes. The specific activities were calculated from the peak areas (n = 8–17, mean ± SD, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, Holm-Sidak’s multiple comparison test for B4GALT1, B4GALT4, CHST1, CHST2, and CHST6, and unpaired t test for B3GNT7).

    Journal: The Journal of Biological Chemistry

    Article Title: Enzymatic basis of branching and extension of O -Man glycans for keratan sulfate biosynthesis

    doi: 10.1016/j.jbc.2026.111140

    Figure Lengend Snippet: Enzyme assays of KS biosynthetic enzymes toward linear and branched O -Man glycans . A , enzymes involved in the KS biosynthesis. B , qPCR analysis of B4galt1 , B4galt4 , B3gnt7 , Chst1 , Chst2 and Chst5 mRNAs in GnT-IX-KO mouse brains (P0) was performed, and their relative levels normalized by that of Gapdh are shown as a graph (n = 3, mean ± SD, ∗ p < 0.05, ∗∗ p < 0.01, unpaired t test). C , soluble B4GALT1, B4GALT4, B3GNT7, CHST1, CHST2, and CHST6 were expressed in HEK293 T cells and purified from the media using a Ni2+ column. The purified enzymes were separated by SDS-PAGE and visualized by CBB staining. D – F , Purified B4GALT1, B4GALT4, B3GNT7, CHST1, CHST2, and CHST6 were incubated with GnM-S-Flu or Gn(Gn)M-S-Flu (for B4GALT1 and B4GALT4) ( D ), GalGnM-S-Flu or GalGn(GalGn)M-S-Flu (for B3GNT7) ( E ), or GnGalGnM-S-Flu or GnGalGn(GnGalGn)M-S-Flu (for CHSTs) ( F ), and the reaction mixtures were analyzed by reverse-phase HPLC. The amount of CHST1 used for GnGalGn(GnGalGn)M-S-Flu was one-20th of the amount for GnGalGnM-S-Flu, while the same amounts of the other enzymes were used for linear and branched substrates. The positions of sulfate residue were predicted by the substrate specificity of the enzymes. The specific activities were calculated from the peak areas (n = 8–17, mean ± SD, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, Holm-Sidak’s multiple comparison test for B4GALT1, B4GALT4, CHST1, CHST2, and CHST6, and unpaired t test for B3GNT7).

    Article Snippet: The primers and probes were purchased from Applied Biosystems as follows: Mm00480752_m1 for B4galt1 Mm00480087_m1 for B4galt4 , Mm01295423_m1 for B3gnt7 , Mm01334076_m1 for Chst1 , Mm00490018_g1 for Chst2 , Mm00517342_m1 for Chst5 , and 4,308,313 (TaqMan Rodent GAPDH Control Reagents) for Gapdh .

    Techniques: Purification, SDS Page, Staining, Incubation, Residue, Comparison

    Actions of KS biosynthetic enzymes on O -Man glycans . B4GALT1, B4GALT4, and CHST1 preferentially act on GnT-IX-produced branched O -Man glycans.

    Journal: The Journal of Biological Chemistry

    Article Title: Enzymatic basis of branching and extension of O -Man glycans for keratan sulfate biosynthesis

    doi: 10.1016/j.jbc.2026.111140

    Figure Lengend Snippet: Actions of KS biosynthetic enzymes on O -Man glycans . B4GALT1, B4GALT4, and CHST1 preferentially act on GnT-IX-produced branched O -Man glycans.

    Article Snippet: The primers and probes were purchased from Applied Biosystems as follows: Mm00480752_m1 for B4galt1 Mm00480087_m1 for B4galt4 , Mm01295423_m1 for B3gnt7 , Mm01334076_m1 for Chst1 , Mm00490018_g1 for Chst2 , Mm00517342_m1 for Chst5 , and 4,308,313 (TaqMan Rodent GAPDH Control Reagents) for Gapdh .

    Techniques: Produced

    FIGURE 1 GM3(Neu5Gc) synthesis and expression in murine cell lines. (A) Schematic overview of the ganglioside biosynthesis pathway. Genes names encoding the enzymes responsible for conversion steps are given in red. Created with BioRender. (B) Drawing illustrating the hydroxylation of N-acetylneuraminic acid (Neu5Ac) to N-glycolylneuraminic acid (Neu5Gc), catalyzed by cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH). (C, D) Histograms showing surface 14F7 scFv staining in wild type (WT) and Cmah -, St3gal5-, and B4galt1 knock-out variants of the murine plasmacytoma cell line X63 and the B lymphoma cell line L1210. (E) Histograms showing 14F7 scFv staining of murine cell lines under standard cell culturing conditions. (F) Histograms showing 14F7 scFv staining of murine colon cancer CT26 and melanoma B16F10 (B16) cells ex vivo isolated from established s.c. tumors (>5mm diameter). (G) 14F7 mAb immunohistochemistry staining of established NS0 and B16 tumors (>5mm diameter). Scale bars indicate 100 µm.

    Journal: Frontiers in immunology

    Article Title: Chimeric antigen receptor T cells targeting the GM3(Neu5Gc) ganglioside.

    doi: 10.3389/fimmu.2024.1331345

    Figure Lengend Snippet: FIGURE 1 GM3(Neu5Gc) synthesis and expression in murine cell lines. (A) Schematic overview of the ganglioside biosynthesis pathway. Genes names encoding the enzymes responsible for conversion steps are given in red. Created with BioRender. (B) Drawing illustrating the hydroxylation of N-acetylneuraminic acid (Neu5Ac) to N-glycolylneuraminic acid (Neu5Gc), catalyzed by cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH). (C, D) Histograms showing surface 14F7 scFv staining in wild type (WT) and Cmah -, St3gal5-, and B4galt1 knock-out variants of the murine plasmacytoma cell line X63 and the B lymphoma cell line L1210. (E) Histograms showing 14F7 scFv staining of murine cell lines under standard cell culturing conditions. (F) Histograms showing 14F7 scFv staining of murine colon cancer CT26 and melanoma B16F10 (B16) cells ex vivo isolated from established s.c. tumors (>5mm diameter). (G) 14F7 mAb immunohistochemistry staining of established NS0 and B16 tumors (>5mm diameter). Scale bars indicate 100 µm.

    Article Snippet: Briefly, the ablation of Cmah, St3gal5, and B4galt1 genes was achieved by electroporating target cells using the Cas9/gRNA vector pSpCas9(BB)-2A-GFP (kindly provided by Dr. Feng Zhang via the Addgene repository; Addgene #48138) with the following guide RNA template sequences: mCmah_s1: 5’- TAGTCGTACCCTCCAGGAAA-3’, mSt3gal5_s1: 5’-TCGGGT GTACCATTGCAGGG-3’, and mB4galt1_s1: 5 ́-CAGGATGCG GCTAGACCGCC-3 ́.

    Techniques: Expressing, Staining, Knock-Out, Cell Culture, Ex Vivo, Isolation, Immunohistochemistry